zymo spin iv filters (Zymo Research)

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Zymo Research zymo spin iv filters
Zymo Spin Iv Filters, supplied by Zymo Research, used in various techniques. Bioz Stars score: 94/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/zymo+spin+filter/Spin-Away+Filters/10__53346_slash_wjapmr__2026__10__2__0013-87-16-16
Average 94 stars, based on 12 article reviews

zymo spin iv filters - by Bioz Stars, 2026-09

94/100 stars

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Extraction:

Article Title: Current Updates on Molecular Diagnostic Assays Used for Detection of Candida auris : A Systematic Review
Article Snippet: Thermal-based , / , / , / , Yeast colonies: (1) suspend the colonies in 100 μL of water; (2) boil at 100 °C for 10 min; (3) centrifuge at 2000 g for 3 min; (4) supernatent then used as PCR template; (5) check DNA purity at A260/A280 using NanoDrop with a ratio of 1.7 to 2.1; (6) 100 pg to 1 μg of genomic DNA is used for PCR amplification.. , [ ] . .. Column-based and bead-based , / , Zymo Quick-DNA Fungal/Bacterial Miniprep kit , Vortex Genie 2 , Manual extraction for isolates: (1) colonies suspended in PBS to yield a concentration of 1 × 10 5 CFU/mL; (2) 200 μL of the sample is vortexed at maximum speed for 10 min with a Vortex Genie 2; (3) spin it down, and filter the supernatent through the Zymo-Spin filter through centrifugation; (4) mix the filtrate with a genomic lysis buffer and then transfer it to a column; (5) final elution volume is 35 μL. , [ ] . .. Chemical-based , 250 μL of sample (1 × 10 5 CFU/mL) mix with 250 μL of binding buffer (4 M guanidine thiocyanate, 0.055 M Tris-HCL pH 7.5, 0.025 M pH 8 EDTA) and 10 μL of silica-coated magnetic beads , / , / , (1) Allow the samples to bind with the binding buffer for 10 min at room temperature; (2) pellet the silica-coated magnetic beads using a handheld magnet; (3) invert the tube to remove the solution; (4) resuspend the beads in 50 μL of wash solution with 0.05% Tween 20; (5) pellet the beads again, and remove the wash solution; (6) repeat for two times; (7) elute the beads in 10 μL of PCR buffer at 60 °C for 5 min. , [ ] .

Article Title: Current Updates on Molecular Diagnostic Assays Used for Detection of Candida auris : A Systematic Review
Article Snippet: Extraction methods , Pre-extraction procedures , Extraction kits used , Equipment used , Protocols , References. .. Column-based and bead-beating , / , Zymo Quick-DNA Fungal/Bacterial Miniprep kit (Zymo Research, Irvine, CA, USA) , Vortex Genie 2 (Scientific Industries, Bohemia, NY, USA) , Manual extraction for isolates: (1) inoculate the colonies in a ZR BashingBead lysis tube with 750 μL of BashingBead buffer; (2) vortex at maximum speed for 10 min with a Vortex Genie 2; (3) spin it down and filter the supernatent through the Zymo-Spin filter through centrifugation; (4) mix the filtrate with genomic lysis buffer and then transfer to a column; (5) final elution volume is 100 μL. , [ ] . .. Column-based and mechanical-based , Add swabs to 1 mL MP Biomedical Lysing Matrix A tube. Then, homogenize the sample for 60 s under the speed of 6 m/s prior to DNA extraction , Qiagen QIAamp DNA Mini Kit (Qiagen, Hilden, Germany) , MP Biomedical FastPrep homogenizer , Semi-automated method: (1) quick spin the homogenized sample and pipette 200 μL into a 2 mL tube containing 20 μL of proteinase K; (2) mix 200 μL Buffer AL with 5 μL internal extraction control; (3) vortex the mixture for 15 s; (4) incubate at 56 °C for 10 min; (5) load the sample tube into the QIAcube and choose the “Body fluid spin protocol”; (6) extraction performed on the QIAcube; (7) final elution volume is 100 μL; (8) 6 μL DNA template for OLM AurisID real-time PCR assay. , This report.

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Article Title: Quantitative Analysis of Genomic DNA Degradation of E. coli Using Automated Gel Electrophoresis under Various Levels of Microwave Exposure
Article Snippet: The supernatant (400 μL) was transferred to a Zymo-Spin filter in a collection tube and centrifuged at 8000× g for 1 min. .. Subsequently, the Zymo-Spin filter was discarded and 1200 μL genomic lysis buffer was added to the filtrate in the collection tube. .. After mixing, 800 μL of the mixture was transferred to a Zymo-Spin IC column in a collection tube and centrifuged at 10,000× g for 1 min. After discarding the flow through from collection tube, 200 μL DNA pre-wash buffer was added to the Zymo-Spin IC column and centrifuged at 10,000× g for 1 min. DNA wash buffer was added to the Zymo-Spin IC column and centrifuged at 10,000× g for 1 min.

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